Evaluation of antimicrobial activities of Harmine, Harmaline, Nicotine and their complexes

Harmine, Harmaline, Nicotine and its various complexes synthesized have been characterized by physical, spectral and analytical methods and curtained for in-vitro antimicrobial activity against different bacterial and fungal species at two different concentrations i.e.100micro g/100micro l and 200micro g/100micro l dose level respectively. Analysis showed that Nicotine, Zinc-Nico, Cd-Nico, Hg-Nico, Ni-Nico, Cu-Nico, Co-Nico, Harmine, and Harmaline having conc. of 100ug/ 100ul had antibacterial activity on zero, 5, 4, 10, zero, 5, 7, zero, zero strain of bacteria having an average of zero [SD=0.0000], 15.2000 [SD=1.30384], 18.2500 [SD=3.30404], 20.2000 [SD=1.39841], zero [SD=0.0000], 14.6000 [SD=0.89443], 15.8571 [SD=1.34519], zero [SD=0.0000], zero [SD=0.0000] respectively. Zinc [II] chloride, Cadmium [II] Iodide, Mercury [II] chloride, Nickel [II] chloride, Copper [II] chloride, Cobalt [II] chloride, Mercury [II] chloride, Mercury [II] harmine, Mercury [II] harmaline at 100ug/100ul is valid for 7, 8, 9, 2, 7, 8, 9, 10, 8 strains of bacteria with an average of 7.1429 [SD=1.06904], 10.0000 [SD=5.01427], 14.8889 [SD=6.00925], 6.0000 [SD=0.0000], 8.5714 [SD=4.27618], 8.2500 [SD=0.88641], 14.8889 [SD=6.00925], 18.6000 [SD=2.45855], 18.5000 [SD=1.85164] respectively. The above given compounds at the conc. of 200 micro g/100ul is valid for 10, 9, 10, 8, 8, 10, 10, 10, 10 strains of bacteria with an average of 8.1 [SD=1.66333], 11.7778 [SD=5.28625], 16.1000 [SD=6.36745], 6.5000 [SD=0.92582], 9.7500 [SD=4.43203], 9.9000 [SD=2.76687], 16.1000 [SD=6.36745], 22.0000 [SD=2.44949], 20.4000 [SD=2.75681] respectively. The above given compounds at conc. of 200 micro g/100ul showed antibacterial action on 3, 8, 8, 10, 3, 9, 8, zero, 3 strains of bacteria with an average of 14[SD=0.000], 16.8750 [SD=1.35620], 18.2500 [SD=3.45378], 22.7000 [SD=1.82878], 14.3333 [SD=0.57735], 16.7778 [SD=1.71594], zero [SD=0.000], 12.0000 [SD=1.00000] respectively. Hence according to the average value of the zone of inhibition, maximum antibacterial activity at 100-200ug/100ul is of Hg-Nico and Mercury salt; Mercury [II] harmine having an average of 20.2000 [SD=1.39841]-22.7000 [SD=1.82878] and 18.6000 [SD=2.45855]-22.0000 [SD=2.44949]. Minimum antibacterial activity at 100-200ug/100ul is Nicotine100, Nicotine-Nico100, Harmine 100,Harmaline 100, Harmine 200 having zero average [SD=0.000]

Refractory myasthenia gravis; patient on rituximab with acute respiratory failuer: case report

Myasthenia gravis [MG] is prototypical neurological autoimmune disorder, characterized by limb and occulo-bulbular fragility. Our case study will facilitate the practitioners as well as the scientific community to consider other therapy plans with patients presented with acute respiratory failure due to the use of rituximab.A 25-year-old man presented with acute respiratory failure This case reporte sheds light on the importance of differential diagnoses of refractory myasthenia gravis for inexplicable acute respiratory failure upon rituximab treatment. The case study demonstrates the problems associated with use of rituximab in refractory myasthenia gravis. It can cause acute respiratory failure among the patients with relapse specially. Our case study will facilitate the practitioners as well as the scientific community to consider other therapy plans with patients presented with acute respiratory failure due to the use of rituximab.